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. 2021 Nov 17;49(21):12348–12357. doi: 10.1093/nar/gkab1002

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — GQ–RGG interaction dynamics monitored at μM concentrations inside a ZMW nanoaperture. (A) Scheme of the experiment. k_on and k_off are the association and dissociation rate constants respectively. (B) Scanning electron microscopy image of a 120 nm ZMW nanoaperture. (C) FCCS curve at different GQs concentrations. The RGG concentration is fixed at 1 μM in all the measurements. Black lines are the FCCS fits according to equation 7. The inset shows the bound fraction of RGG peptide as a function of GQs concentration together with a Hill equation fit (Eq. 10) to determine the dissociation constant. (D) Amplitude S and time scale τ_S of the interaction dynamics as a function of GQs concentration. (E) Association k_on.[GQ] and dissociation k_off rate as a function of GQs concentration.