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. 2021 Nov 17;49(21):12348–12357. doi: 10.1093/nar/gkab1002

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Influence of salt concentrations and DNA sequence. (A) FCCS curve at different KCl concentrations. The RGG and GQ concentrations are 1 and 40 μM, respectively. The inset shows the bound fraction of RGG peptide. (B) Amplitude S and characteristic time τ_S as a function of KCl concentration. (C) Association k_on.[GQ] and dissociation k_off rate as a function of KCl concentration. (D) FCCS curves obtained for different DNA samples: Alexa 546 labelled G-quadruplex (GQ-A546), Cy3B labelled G-quadruplex (GQ-Cy3B), Alexa 546 labelled 51 bases DNA single strand (ssDNA-A546), and Alexa 546 labelled 51 bp DNA double strand (dsDNA-A546). (E) S and τ_S for the different DNA samples. (F) Association k_on.[GQ] and dissociation k_off rates for different DNA samples.