Figure 1.

The concept of CRISPR-induced chromosomal deletions. (A) Schematic overview of chromosome 11. Scissors represent the gRNA locations and green and red colors the regions adjacent to the gRNAs. DSBs could lead to joining of the green and red region with loss of the intermittent region. (B) Copy number profile of chromosome 11q of the neuroblastoma cell line GIMEN. Data was generated by WGS and visualized using the R2 bioinformatics platform. Black dots represent values for bins and red dots represent segmented values. The red arrows represent the gRNA location at chromosome 11q13.4and near the telomere at 11q25. Ideograms representing the chromosomal location and banding pattern (centromeres are represented in yellow) are shown above the profile.