Table 1.

T _m and ΔT_m/mod data from UV-melting curves (260 nm) of ON1-5 in duplex with complementary DNA and RNA.

Entry	Sequencea,b,c	T m versus DNA [°C]	ΔTm/mod [°C]	T m versus RNA [°C]	ΔTm/mod [°C]
ON1	5′-d(GGA TGT TCt CGA)-3′	40.0	-9.1	42.6	-6.8
ON2	5′-d(GGA t GT TCT CGA)-3′	43.0	-6.1	45.8	-3.6
ON3	5′-d(GGA tGT TCt CGA)-3′	32.8	-8.1	38.0	-5.7
ON4	5′-d(GGA TGt tCT CGA)-3′	42.9	-3.1	47.0	-1.2
ON5	5′-d(GCA ttt ttA CCG)-3′d	34.0	-2.7	37.2	-1.4

^aTotal strand conc. 2 μM in 10 mM NaH₂PO₄, 150 mM NaCl, pH 7.0.

^bA, G, T, C denote natural 2‘-deoxynucleosides; t corresponds to modified nucleosides.

^c T _m of unmodified duplexes, DNA/DNA: 49.1°C, DNA/RNA: 49.4°C, RNA/RNA: 60.3°C.

^d T _m of unmodified duplexes, DNA/DNA: 47.5°C, DNA/RNA: 44.0°C.