Table 3.

T _m values from UV-melting curves (260 nm) of ON7-8 and Tc1 in duplex with ON6.

Entry	Sequencea,b	NaCl concentration [mM]	ΔTm versus ON6c [°C]
ON7	5’-d(cct aca aga gct)-7′	150	83.6
ON7	5′-d(cct aca aga gct)-7′	50	79.6
ON7	5′-d(cct aca aga gct)-7′	0	68.6
ON8	5′-d(tcg aga aca tcc)-7′	150	<10
Tc1	5′-d(tcg agaacatcc)-3′	150	81.0

^aTotal strand conc. 2μM in 10 mM NaH₂PO₄, pH 7.0.

^b a, g, t, c corresponds to modified adenine, guanine, thymine and methylcytosine, respectively, a, g, t, c corresponds to tricyclo-DNA adenine, guanine, thymine and methylcytosine, respectively.

^c ON6 sequence: 5′-d(agc tct tgt agg)-7′.