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. 2021 Sep 23;49(21):e123. doi: 10.1093/nar/gkab791

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Frequencies of promoter and RBS parts in pathway variants. All 40 pathway variants used to transform Synechocystis were sequenced and the promoter and RBS upstream of each CDS were identified. (A) Schematic of construct library variants. (B) Relative frequencies of promoter and RBS parts: In segregated pathway variants, black bars; in non-segregated pathway variants, grey bars. Promoters, in descending order of strength as characterised using EYFP: P1 = SPLc25, P2 = SPLc19, P3 = SPLc47, P4 = SPLc3, P5 = SPLc45 and P6 = SPLc17. RBSs, in descending order of strength as characterised using EYFP: R1 = RBSc4, R2 = RBSc21, R3 = RBSc110, R4 = RBSc123, R5 = RBSc48 and R6 = RBSc249.