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. 2021 Nov 25;49(21):12377–12393. doi: 10.1093/nar/gkab1123

Figure 3.

Figure 3.

Telomeric duplex bound TRF2 enhances POT1-overhang dynamics. (A) Schematic model of telomeric duplex (four repeats of TTAGGG tract) with G4 overhang sequentially binding POT1 at the overhang followed by TRF2 at the duplex. The basic domain of TRF2 can bind to ss-ds junction, for simplicity isn’t shown here. (B) The FRET histograms of telomeric duplex with G4 (∼0.65 FRET, black solid line), POT1 bound condition (∼0.3 FRET, orange solid line), and POT1, TRF2 together (∼0.3 FRET, solid blue filled). (C) POT1 and TRF2 bound representative smFRET traces of Dynamic-I (top) and Dynamic-II (bottom) states. (D) Quantification of molecular behavior (steady vs two types of dynamics) of 4R bound POT1 in presence and absence of TRF2. All statistics for this figure are calculated using a two-tailed two-sample Student's t test (*P < 0.05; **P < 0.01). (E) Representative smFRET trace of real time TRF2 binding at telomeric duplex (flow at ∼10 s) contain POT1 pre-bound overhang. (F) The heatmap (n > 100) of POT1 bound state in absence (top) and presence (bottom) of TRF2 (flow at ∼10 s).