. 2021 Nov 25;49(21):12069–12088. doi: 10.1093/nar/gkab1126

Table 1.

Sequence of ⁱE-VP modified guide strands and control guide strands

^VPGuide strands	Sequence (5′ → 3′)^a	Calcd. mass [M-H]^–	Observed mass	Correspond Passenger strands^b	^VPsiRNA #	Correspond ^CtrlsiRNA^c #
^VPG1	P-UvpU#AAUCUCUUUAC#U#G#A#U#A#U#A	6599.4	6599.7	P1	^VPD1	^CtrlD1
^VPG2	P-U#UvpUUUAAAUCCUG#A#G#A#A#G#A#A	6724.5	6724.8	P2	^VPD2	^CtrlD2
^VPG3	P-U#U#UvpUUAAAUCCUG#A#G#A#A#G#A#A	6739.6	6740.7	P2	^VPD3	^CtrlD2
^VPG4	P-U#U#UUvpUAAAUCCUG#A#G#A#A#G#A#A	6740.6	6740.7	P2	^VPD4	^CtrlD2
^VPG5	P-U#C#UCUvpUUACUGAU#A#U#A#A#U#U#A	6614.4	6615.7	P3	^VPD5	^CtrlD3
^VPG6	P-U#A#UGUUvpUUCACAU#A#U#U#G#U#C#A	6631.4	6631.6	P4	^VPD6	^CtrlD4
^VPG7	P-U#G#AAUGUvpUCACGC#A#G#U#G#G#G#C	6802.6	6802.8	P5	^VPD7	^CtrlD5
^VPG8	P-U#A#UCAGCUvpUUUCC#A#G#G#G#U#C#G	6700.5	6700.7	P6	^VPD8	^CtrlD6
^VPG9	P-U#U#AAUCUCUvpUUAC#U#G#A#U#A#U#A	6615.4	6615.7	P1	^VPD9	^CtrlD1
^VPG10	P-U#U#AAUCUCUUvpUAC#U#G#A#U#A#U#A	6615.4	6615.7	P1	^VPD10	^CtrlD1
^VPG11	P-U#U#AACGUCAGUvpUC#A#U#A#A#A#C#C	6675.5	6675.7	P7	^VPD11	^CtrlD7
^VPG12	P-U#C#CACUAUGUUUvpU#C#A#C#A#U#A#U	6590.4	6590.7	P8	^VPD12	^CtrlD8
^VPG13	P-U#C#CAAAUACUGGUvpU#G#U#C#G#G#U	6708.5	6708.7	P9	^VPD13	^CtrlD9
^VPG14	P-U#C#CGGUCACAACA#UvpU#G#U#G#G#U	6707.5	6707.7	P10	^VPD14	^CtrlD10
^VPG15	P-U#A#UGUUUUCACAU#A#UvpU#G#U#C#A	6615.4	6615.7	P14	^VPD15	^CtrlD4
^VPG16	P-U#U#UGGUAGCUGAA#A#G#UvpU#C#U#U	6710.4	6710.7	P11	^VPD16	^CtrlD11
^VPG17	P-U#U#AAUCUCUUUAC#U#G#A#UvpU#U#A	6576.3	6576.6	P1	^VPD17	^CtrlD12
^VPG18	P-U#C#UCUUUACUGAU#A#U#A#A#UvpU#A	6599.4	6599.7	P3	^VPD18	^CtrlD3
^VPG19	P-U#U#AAUCUCUUUAC#U#G#A#U#A#UvpU	6576.3	6576.7	P1	^VPD19	^CtrlD13
^VPG20	P-UvpU#AAUCUCUUUAC#U#G#A#UvpU#UvpU	6513.2	6513.7	P1	^VPD20	^CtrlD14

^aUppercase and underlined uppercase represent 2′-OMe and 2′-F, respectively. 5′-end phosphate is represented as ‘P’. Inter-nucleotide phosphorothioate and (E)-vinylphosphonate linkages are indicated by ‘#’ and vp, respectively.

^b3′-end tetraethylene glycol (Teg) -linked cholesterol (Chol) conjugated 15 mer passenger strands (P1–P14) to prepare ^VPsiRNAs (^VPD1–^VPD20) consists with (i) complementary sequence through 5′-end to 15th nucleotide of guide strands and (ii) with fully chemical modification pattern: 5′-N#N#NNNNNNNNNNN#N#N-Teg-Chol-3′ (N and N represent 2′-OMe and 2′-F, respectively. See supporting information for each of passenger strand sequences).

^cComparative control siRNA duplexes corresponding to each ^VPsiRNA. Sequences shown in supporting information.