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In vitro 1A3-MMAE cytotoxicity in MCF-7 (A) and MCF-7F (B) cells measured by MTT assay after treatment with 1A3-MMAE, 1A3 or MMAE. C. Clonogenic growth of MCF-7 or MCF-7F cells after exposure to 1A3-MMAE and replating. D. The quantification of clonogenic growth assays in C. E. Tubulin staining of MCF-7 cells after 48 h treatment with MMAE, 1A3, 1A3-MMAE (40× magnification).
