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. 2000 Apr;38(4):1324–1330. doi: 10.1128/jcm.38.4.1324-1330.2000

FIG. 4.

FIG. 4

Restriction enzyme profiles of SSU rDNA digested with HinfI (A) and RsaI (B). The HinfI restriction enzyme produced five patterns, while the RsaI restriction product showed four kinds of patterns. Strains HJ96A-26, HJ96B-1, HJ96B-4, HJ96B-6, and HV93-13 showed the same RFLP pattern with both HinfI and RsaI enzymes. HJ96A-29 strain showed the same RFLP pattern with Nand II and HE87-1 strains with RsaI enzyme, while with HinfI enzyme the HJ96A-29 strain showed two bands of 180 and 100 bp instead of a band of 280 bp as with the Nand II and HE87-1 strains. Strains HJ96AS-1 and B also showed distinct banding patterns. Lanes: MM, molecular marker of a 100-bp ladder; 1, HJ96A-26; 2, HJ96A-29; 3, HJ96AS-1; 4, HJ96B-1; 5, HJ96B-4; 6, HJ96B-6; 7, HV93-13; 8, Nand II; 9, HE87-1; 10, B strain.