Figure 1.

GPI-lipid remodeling in yeast. The GPI anchor is synthesized in the ER and consists of a glycan core and phosphatidylinositol (PI) with an acyl chain at the 2-position of the inositol ring (Kinoshita and Fujita, 2016). After protein attachment, the glycan and lipid moieties are remodeled and this process has been shown to be very important for the transport and final localization of the GPI-APs. The GPI-lipid undergoes a structural remodeling that has the purpose of providing saturated lipids and in yeast it occurs almost entirely at the ER where it is initiated with the inositol deacylation at the 2-position of the inositol ring by the remodeling enzyme Bst1p (PGAP1 in mammals). The rest of lipid remodeling enzymes are indicated, see text for details (modified from Muniz and Zurzolo, 2014). IPC, inositolphosphoceramide.