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. 2021 Aug 16;187(4):2156–2173. doi: 10.1093/plphys/kiab384

Figure 7.

Figure 7

Localization of GFP-AGP4 and GFP-GPI in wild-type and pgap1–1 Arabidopsis protoplasts. Transient expression in wild-type (A–C) and pgap1–1 Arabidopsis protoplasts (D–X). A–F, GFP-AGP4 (A and B) and GFP-GPI (C) localized in the plasma membrane in wild-type protoplasts. A and B show two different confocal planes In contrast, GFP-AGP4 (D, E) and GFP-GPI (F) showed an ER-like localization pattern in pgap1–1 protoplasts (D–F). G–L, Coexpression of GFP-AGP4 (G) or GFP-GPI (J) with the ER marker RFP-calnexin (H and K; see merged images in I and L). M–R, Coexpression of GFP-AGP4 (M) or GFP-GPI (P) with the ER marker RFP- p24δ5 (N and Q; see merged images in O and R). S–X, Colocalization of GFP-AGP4 (S) or GFP-GPI (V) with the FM dye FM4–64 (T, W; see merged images in U and X). Scale bars = 10 μm.