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. 2021 Sep 15;187(4):2352–2355. doi: 10.1093/plphys/kiab423

Figure 2.

Figure 2

A 27°C pulse inhibits the association of ELF3 to foci in root nuclei. The localization of ELF3 at dusk in root nuclei of (A and B) 35S::YFP:ELF3 elf3-4 (wild type) or (C and D) 35S::YFP:ELF3 elf3-4/elf4-1 (elf4 mutant). Images were taken at (A and C) 22°C or (B and D) after a 2-h 27°C pulse started at ZT6 (short-day 8/16 photoperiods). E, Number of foci per nuclei under the respective treatment. F, Relative change in the number of ELF3 foci following a temperature pulse in the elf3-4 or elf3-4/elf4-1 background. Data were relative to the respective genotype at 22°C. Images were collected on two occasions, with a minimum of 10 images analyzed in total. For both (E and F) the center line within the box defines the median, with the cross defining the mean. The limits of the box define the upper and lower quartiles, while the whiskers extend 1.5× the interquartile range. Significance was determined by a T test: n.s = no significance, ***P < 0.001. Scale bars are 5 μm.