TABLE 2.

Primer sequences for PCR and sequencing of 16S rRNA, 444 Ep-ank and groESL heat shock operon genes of the E. phagocytophila genogroup

Gene and primer name	Functions	Nucleotide sequences of primers (5′–3′)	Source or reference
16S rRNA
EE1	PCR, outer primer set	TCCTGGCTCAGAACGAACGCTGGCGGC	4
EE2		AGTCACTGACCCAACCTTAAATGGCTG
EE3	PCR, inner primer set	GTCGAACGGATTATTCTTTATAGCTTGC
EE4		CCCTTCCGTTAAGAAGGATCTAATCTCC
EE5F	PCR, sequencing, end part of 16S rRNA primer set (517 bp)	ACCTTACCACTCCTTGACATGG	8)
EE6R		CACCCTAGTCACTGACCCAA
EE7F	Sequencing, internal primers	AATTATTGGGCGTAAAGGGCA
EE8R		TTAAGCCCTGGCATTTCACC
444 Ep-ank
LA6	PCR, sequencing	GAGAGATGCTTATGGTAAGAC	Caturegli et al., submitted
LA1		CGTTCAGCCATCATTGTGAC
groESL heat shock operon
EEgro1F	PCR	GAGTTCGACGGTAAGAAGTTCA	Newly designed primers
EEgro2R		CAGCGTCGTTCTTACTAGGAAC
EEgro3F	Sequencing, internal primers	GCGAATGGAGACAAGAACATA
EEgro4R		AGTTGCGTCTTTTGTGATTCG
EEgro5F		AGCGAAGTTGAGGTGAAGGA
Eegro6R		CGCTTCCTTAGCCTTGAGAA