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. 2021 Feb 24;187(4):2192–2208. doi: 10.1093/plphys/kiab099

Figure 1.

Figure 1

Characterization of the gpa8 mutant. A, Images of WT (japonica variety Ninggeng 1), gpa8-1 and gpa8-2 seedlings grown for 6 d. Bars = 1 cm. B, Transverse sections of representative WT and gpa8-1 mutant dry seeds. Bars = 1 mm. C and D, Scanning electron microscopy images of transverse sections of WT (C) and gpa8-1 mutant (D) seeds. Bars = 10 μm. E, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of total seed storage proteins of WT and the gpa8-1 mutant. pGlu, 57-kDa proglutelins; αGlu, 40-kDa glutelin acidic subunits; αGlb, 26-kDa α-globulin; βGlu, 20-kDa glutelin basic subunits; Pro, prolamins. F, Immunoblot analysis of glutelins and molecular chaperones BiP1 and PDI1-1. Arrowhead represents the glutelin basic subunits. Arrows indicate the 57-kDa proglutelins (red) and the glutelin acidic subunits (black). EF-1α was used as the loading control.