Figure 5.

Map-based cloning of GPA8. A, Fine mapping of the GPA8 locus. Molecular markers and numbers of recombinants are shown. B, Gene structure and the mutation site in LOC_Os01g46980 comprising six exons (closed boxes) and five introns (lines). ATG and TGA represent the start and stop codons, respectively. C–F, LOC_Os01g46980 coding sequence under the control of maize ubiquitin promoter rescues grain appearance (C), the storage protein composition pattern (D), the ultrastructures of endosperm cells in the WT (E), and L1 (F). Irregular and round shape protein bodies indicate PBIIs and PBIs, respectively. CW, cell wall. L1–L3 denote grains from three independent T₁ transgenic lines. Red arrow indicates the 57-kDa proglutelins. Bars = 1 mm in (C). Bars = 2 μm in (E) and (F).