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GPA8 encodes a functional subunit E1 of vacuolar H+-ATPase. A and B, Mass spectrometry analysis of the rice V-ATPase complexes. A, Coomassie brilliant blue-stained SDS–PAGE of p35S::GFP transgene (left lane) and p35S::OsVHA–GFP transgene immunoprecipitates (right lane). B, Some OsVHA-E1-interacting proteins identified by mass spectrometry analysis. C, V-ATPase activity. Three biological replicates were performed, values are means ± sd. **P <0.01; Student’s t test.
