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. 2021 Feb 24;187(4):2192–2208. doi: 10.1093/plphys/kiab099

Figure 8.

Figure 8

OsVHA-E1 regulates pH of TGN and vacuole. A, In vivo calibration curve of pH. pH calibration was achieved by equilibrating intracellular pH with 10 µM nigericin, 60-mM KCl, and 10-mM MES/HEPES Bis–Tris–propane, pH 5.0–8.0 (means ± sd; n ≥25 protoplasts). B and C, Representative pseudocolored images of PRpHluorin-BP80 (Y612A) in WT (B) and gpa8-1 (C) protoplasts. Bars = 5 μm. D, pH of the TGN (means ± sd; n ≥ 25 protoplasts; **P < 0.01; Student’s t test). E, In vivo calibration curve of pH of BCECF-AM dye. pH equilibration buffers contain 50-mM Mes-BTP (pH 5.0–6.5) or 50-mM HEPES-BTP (pH 7.0–7.5) and 50-mM ammonium acetate (means ± sd; n ≥ 15 roots). F, Vacuolar pH (means ± sd; n ≥ 15 roots; **P < 0.01; Student’s t test).