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Subcellular localization of GPA1 and GPA3 are altered in gpa8-1. A and B, Localization of GFP-GPA1 in WT (A) and gpa8-1 (B) protoplasts. C and D, Localization of GPA3-GFP in WT (C) and gpa8-1 (D) protoplasts. mRFP-SYP61 serves as a TGN marker. Bars = 5 μm in (A–D). E and F, PSC of GPA1 (E) and GPA3 (F) with mRFP-SYP61. Values are means ± sd. **P <0.01 (n = 45 protoplasts, Student’s t test).
