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. 2021 Sep 21;187(4):2865–2876. doi: 10.1093/plphys/kiab443

Figure 1.

Figure 1

Schematic representation of viral constructs derived from CMV. A, Schematic representation of the full-length cDNA clones of CMV. pCMVZ1, pCMVZ2, and pCMVZ3 were constructed from CMV-ZMBJ, whereas pCMVF1, pCMVF2, and pCMVF3 were from CMV-Fny. pCMVFZ3 and pCMVZF3 were two hybrid constructs. CMV genome encodes four proteins: 1a, 2a, MP, and coat protein (CP). Each vector contains double Cauliflower mosaic virus (CaMV) 35S promoters (2 × 35S, indicated by arrow heads) and a ribozyme sequence (Rz) derived from Tobacco ring spot virus (TRSV). B, The organization of pCMVZ22bN81 (Wang et al., 2016) for use in Pr CMV VIGS system. The MCS was used for the insertion of target gene. C, The organization of pCMVZ22bN81-LIC for use in Pr CMV-LIC-based VIGS system. The LIC site was used for the insertion of target gene by LIC approach (Dong et al., 2007). Nucleotide sequences underlined represent the LIC adaptors.