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. 2021 Sep 21;187(4):2865–2876. doi: 10.1093/plphys/kiab443

Figure 3.

Figure 3

Silencing of the ZmIspH gene in maize by Pr CMV VIGS. A and B, Phenotypes of maize plants infected with Pr CMV::ZmIspH or Pr CMV::LUC control. Photographs were taken at 14 dpi for the first systemic leaf (1SL), at 21 dpi for the 2SL, at 28 dpi for the 3SL, at 35 dpi for the 4SL, at 42 dpi for the 5SL, at 49 dpi for the 6SL, at 56 dpi for the 7SL and at 105 dpi for the 13SL, respectively. Scale bars are 2 cm in (A) and 10 cm in (B). C, RT-qPCR quantification of relative mRNA levels of ZmIspH gene in maize infected with Pr CMV::ZmIspH or Pr CMV::LUC control. Relative mRNA levels of ZmIspH were normalized against those of elongation factor 1-alpha (EF1α) as the reference gene. The bars represent the mean ± SE from at least three independent experiments. In all panels, black asterisks denote significant differences with respect to the control plants infected with Pr CMV::LUC determined by Student’s t test at ***P < 0.001. D, RT-PCR analysis of CP from leaves of maize infected with Pr CMV::ZmIspH or Pr CMV::LUC control. Plant EF1α mRNA was used as the internal control. Lane M is the 2 kb plus DNA marker.