Figure 4.

Genetic stability of gene insertion of ZmIspH in upper infected with Pr CMV::ZmIspH. Leaf samples were collected at 14 dpi for the first systemic leaf (1SL), at 21 dpi for the 2SL, at 28 dpi for the 3SL, at 35 dpi for the 4SL, at 42 dpi for the 5SL, at 49 dpi for the 6SL, at 56 dpi for the 7SL, and at 105 dpi for 13SL, respectively. Total RNA was isolated from the samples and subjected to RT-PCR amplification with primers flanking the insertion of ZmIspH (upper panel). The vector plasmid with the ZmIspH insert or no sequence insertion was amplified as the positive (lane PC) or negative (lane NC) control. CP was amplified and used as an internal control (lower panel). Lane M is the 2 kb plus DNA marker.