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. 2021 Sep 21;187(4):2865–2876. doi: 10.1093/plphys/kiab443

Figure 5.

Figure 5

Silencing of the ZmIspH, ZmChlH, and ZmPDS genes in maize by Pr CMV-LIC VIGS vectors. A, Leaf phenotype of maize infected with Pr CMV-LIC::ZmIspH, Pr CMV-LIC::ZmChlH, Pr CMV-LIC::ZmPDS, or Pr CMV-LIC::LUC control. The second systemic leaf (2SL) was photographed 21 dpi for each construct. Scale bars are 2 cm. B, RT-qPCR quantification of relative mRNA levels of target genes in maize infected with Pr CMV-LIC VIGS vectors. Relative mRNA levels of target gene (ZmIspH, ZmChlH, or ZmPDS) were normalized against those of EF1α as the reference gene. The bars represent the mean ± SE from at least three independent experiments. In all panels, black asterisks denote significant differences with respect to the control plants infected with Pr CMV-LIC::LUC determined by Student’s t test at ***P < 0.001. C, RT-PCR analysis of CP (upper panel) from leaves of maize infected with Pr CMV-LIC VIGS vectors. Plant EF1α mRNA was used as the internal control. Lane M is the 2 kb plus DNA marker.