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. 2021 Dec 4;16:24. doi: 10.1186/s13062-021-00312-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — YY1 is the target gene of miR-3614-5p in HCC cells. A The expression of candidate mRNAs was tested through qRT-PCR in miR-3614-5p mimics transfected cells. B The expression of YY1 in HCC cell lines and normal cell line was detected via qRT-PCR. C RIP assay verified the existence of LOXL1-AS1/miR-3614-5p/YY1 in RISC complex. D RNA pull down assay further proved the interaction of miR-3614-5p and YY1. E The binding sites of miR-3614-5p and YY1 were demonstrated. F Luciferase reporter assay proved the combination of miR-3614-5p and YY1 by measuring the luciferase activity of YY1-WT/Mut under the influence of miR-3614-5p mimics transfection. **P < 0.01