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. 2021 Dec 5;11:199. doi: 10.1186/s13578-021-00720-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The primary screening of a natural product library. a A general view for the primary screening. The red dashed line in the figure indicated 50% inhibition, and the inhibitory activity of candidate compound was higher than this defined red line. b The chemical structure of dieckol, a natural phlorotannin component extracted from Ecklonia cava. c The inhibitory activity of dieckol in the FP screening assay. The inhibitory analysis of dieckol in the absence or presence of 1 mM DTT was performed as described for GC-376. The IC₅₀ value of dieckol was analyzed using GraphPad Prism 5.0. All assays were performed in triplicate. d Analysis of interaction between dieckol and Mpro using the SPR assay. The binding kinetics of dieckol (0, 6.25, 12.5, 25 µM) to immobilized Mpro was plotted according to the change of RIU value