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. 2021 Dec 5;11:199. doi: 10.1186/s13578-021-00720-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — The inhibition mechanism of dieckol. a The inhibitory activity of dieckol in the FRET assay. Mpro (0.4 µM) was preincubated with the indicated concentrations of dieckol at RT for 35 min in the presence of 1 mM DTT in FP assay buffer. After addition of FRET substrate, the RFU value was separately measured every second for 3 min by a microplate reader (BioTek). The V_I was calculated by the slope of a linear regression in the first 30 s. DMSO and GC-376 (1 µM) was used as the negative and positive control, respectively. The RFU value was notably quenched by dieckol in a dose-dependent manner. b The inhibitory activity of dieckol in the absence or present of DTT. The IC₅₀ values were separately obtained from dose-response curves by plotting the V_I against various concentrations of dieckol in GraphPad Prmis 5.0. c The Lineweaver–Burk plots for analysis the inhibition mechanism of dieckol on Mpro using a FRET assay. d The secondary plots for a K_i value of dieckol in the FRET substrate