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. 2021 Nov 15;134(22):jcs258834. doi: 10.1242/jcs.258834

Fig. 2.

Fig. 2.

PQN-59 and GTBP-1 granules form in response to several stresses in the embryo and the germline. (A) Epifluorescence microscope images of pqn-59::GFP;gtbp-1::RFP perm-1(RNAi) embryos (ctrl) and perm-1(RNAi) embryos treated with arsenite, or arsenite and cycloheximide as indicated on the left and (B) quantifications of the percentage of embryos with granules (ctrl, n=42, arsenite, n=143, arsenite and cycloheximide, n=87, N=2). (C) Epifluorescence microscope images of germlines of pqn-59::GFP;gtbp-1::RFP from control and worms treated with arsenite or puromycin, as indicated on the left. (D) Quantifications of the germline showing granules (ctrl, n=53, arsenite, n=51, puromycin, n=58, N=4). ROIs are shown enlarged on the right. Error bars indicate s.d. n indicates the number of samples and N the number of independent experiments.The P-value was determined using one-way ANOVA followed by a Tukey's multiple comparisons test. Scale bars: 10 μm.