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Interexperimental variability: viral load in five separate aliquots of PBMCs from a patient with MCD
| DNA extract | No. of HHV-8 DNA copies per 106 cells in aliquot:
|
CVa (%) | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | Mean | SD | ||
| Undilutedb | 1,209,724 | 1,291,996 | 1,368,835 | 1,243,433 | 1,343,713 | 1,291,540 | 66,530 | 5.2 |
| 1:10 dilutionb | 1,445,277 | 1,421,853 | 1,463,155 | 1,276,061 | 1,119,156 | 1,345,100 | 146,356 | 10.9 |
CV, coefficient of variation.
Each measurement was carried out in duplicate.
