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ALKBH5 is lowly expressed in ESCC tissues. The mRNA (a) and protein (b) expression of ALKBH5 in 23 pairs of ESCC and adjacent normal tissue samples using qRT-PCR and IHC. (c) The data statistics of IHC. (d) The total m6A levels in ESCC and normal tissues by ELISA. (e) ALKBH5 protein was significantly overexpressed in Eca-109 and KYSE150 cells following transfection of the pcDNA3.1-ALKBH5 plasmid (OE). NC: negative control. P < 0.05.
