Fig. 2.

Heatmap representing the pH profiles for the purified enzymes. Abbreviations as in Fig. 1. The data represent the relative percentages of specific activity, determined as follows: [protein], 0.002–0.04 mg ml⁻¹; [substrate], 0.3 mg ml⁻¹; pH, 3.0–9.0; T, 30 °C; reaction volume, 200 μl. The plot was made with heatmap3 R package version 1.9.1 [55]. The raw data are available in Table S5.