Figure 1.

circPTEN expression is significantly downregulated in CRC tissues and cells

(A) qRT-PCR analysis of circPTEN expression in CRC patients at different stages. (B) circPTEN expression in non-metastatic CRC tissues and metastatic CRC tissues was detected via qRT-PCR. (C) Analysis of the overall survival of CRC patients with low or high expression of circPTEN is shown. (D) qRT-PCR analysis of circPTEN expression in CRC cell lines and HCoEpiC cells is shown. (E) The genomic location and splicing pattern of hsa_circ_0094343 (circPTEN) is shown. (F) Nucleic acid electrophoresis showed that divergent primers could produce the circular isoform of PTEN with cDNA, but not with gDNA. GAPDH was an endogenous control. (G) The resistance of circPTEN and PTEN to ActD was analyzed in RKO and SW620 cells. (H) The expression of circPTEN and PTEN mRNA was examined via qRT-PCR in RKO and SW620 cells after RNase R treatment. (I) Detection of the subcellular localization of circPTEN through FISH (bar value = 20 μm) assay is shown. ∗∗p < 0.01. n.s., no significance.