Figure 2.

Upregulation of circPTEN restrains malignant behaviors of CRC cells

(A) qRT-PCR analysis of circPTEN overexpression efficiency in RKO and SW620 cells. (B and C) Evaluation of proliferation of transfected cells via colony formation and EdU (bar value = 150 μm) assays is shown. (D) Measurement of the apoptosis of transfected cells through TUNEL (bar value = 150 μm) assay is shown. (E and F) Analysis of the migratory and invasive capabilities of transfected RKO and SW620 cells via wound healing (bar value = 100 μm) and transwell (bar value = 70 μm) assays is shown. (G) Western blot analysis of EMT-related proteins in different groups is shown. (H) IF (bar value = 20 μm) analysis of E-cadherin and N-cadherin in transfected cells is shown. ∗∗p < 0.01.