Figure 3.

circPTEN regulates PTEN expression by sequestering miR-4470 in CRC cells

(A) qRT-PCR analysis of PTEN expression in RKO and SW620 cells with circPTEN overexpression. (B) The enrichment of circPTEN in the immunoprecipitates conjugated to anti-Ago2 was detected via qRT-PCR in RIP assay. (C) Six miRNAs were predicted to bind with circPTEN and PTEN via miRDB. (D) The coexistence of circPTEN, miR-4470, and PTEN in RISC was testified via Ago2-RIP assay. (E) The binding capacity between miR-4470 and circPTEN (or PTEN) was validated via RNA pull-down assay. (F) The binding sites between miR-4470 and circPTEN (or PTEN) were predicted through miRDB. (G) The interaction between circPTEN and miR-4470 was demonstrated by luciferase reporter assay. (H) The competitive binding relationship among circPTEN, miR-4470, and PTEN was verified through luciferase reporter assay. ∗∗p < 0.01.