Figure 4.

circPTEN mediates malignant behaviors of CRC cells via AKT signaling pathway

(A) Western blot analysis of protein expression in cells with circPTEN upregulation. (B) The knockdown efficiency of sh-PTEN was evaluated via qRT-PCR and western blot. (C) Western blot analysis of protein expression after transfection with different plasmids is shown. (D and E) Evaluation of cell proliferation in different groups via colony formation and EdU (bar value = 150 μm) assays is shown. (F) TUNEL (bar value = 150 μm) assay was performed to measure cell apoptosis in different groups. (G and H) Analysis of the migration and invasion abilities via wound healing (bar value = 100 μm) and transwell (bar value = 70 μm) analyses is shown. (I and J) EMT markers in different groups were detected via western blot and IF (bar value = 20 μm). ∗∗p < 0.01.