Figure 5.

circPTEN regulates K63-linked ubiquitination of AKT through TRAF6 in CRC cells

(A) TRAF6 was revealed to bind with circPTEN through RNA pull-down and mass spectrometry. (B) The binding capacity between circPTEN and TRAF6 was proven by RNA pull-down and RIP assays. (C) The structure of TRAF6 is shown. (D) TRAF6 was divided into 12 segments. (E and F) The binding of circPTEN to the Ring finger domain of TRAF6 was testified via RIP and RNA pull-down assays. (G) Verification of the binding between circPTEN and the Ring finger domain of TRAF6 via RIP is shown. ∗∗p < 0.01.