Figure 6.

circPTEN inhibits the phosphorylation of AKT in CRC cells

(A) coIP assay confirmed that circPTEN could repress the binding between TRAF6 and AKT. WCEs were collected for immunoprecipitation. WB referred to western blot and NB represented Northern blot. (B) TRAF6 expression in transfected cells was detected via qRT-PCR and western blot analysis. (C) The effect of circPTEN on the interaction between TRAF6 and AKT was proven via coIP. (D) Western blot analysis of protein expression is shown.