Figure 7.

Upregulation of circPTEN inhibits CRC progression via PTEN/AKT pathway

(A–C) Transfected RKO cells were subcutaneously injected into nude mice, and later, tumor growth, volume, and weight were analyzed. (D) qRT-PCR analysis of circPTEN or PTEN expression in different groups is shown. (E) Western blot analysis of AKT or p-AKT expression in different groups is shown. (F) IF (bar value = 80 μm) analysis of proteins (Ki67, PTEN, AKT, p-AKT, E-cadherin, and N-cadherin) in different groups is shown. (G) H&E (bar value = 100 μm) staining was utilized for evaluation of metastasis in different groups. ∗∗p < 0.01.