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. 2021 Nov 22;12:769699. doi: 10.3389/fgene.2021.769699

FIGURE 3.

FIGURE 3

Disruptions of ABCG5/8 Genes Mimicked the Phenotypes of the Proband in the Double Knockout Rats. Panel (A) shows the strategy for the generation of ABCG5/8 double knockout using the CAS9/sgRNA system. Panel (B) showed significantly increased blood lipid levels (left) and the large platelet (right) in the homozygotes compared with two other groups. For each group, the blood lipid levels and the large platelet were determined from randomly selecting rats. The values of the blood lipid levels are expressed as the mean ± SE. n = 6. *p < 0.05, **p < 0.01. The large platelet was detected in over 80% of homozygotes, and none was found in the wild-type strain. Panel (C) shows a blood smear for three groups of the rat model.