FIGURE 4.

T cells promote EBV transformation through humoral factors. A, B cells isolated from PBMCs were infected with Akata EBV and cultured for 3 wk in medium containing DMSO or C‐176 (0.5 µM). Wells with transformed cells were counted within a 96‐well plate to calculate the EBV transformation efficiency (TD₅₀/mL). This assay was also performed under B and T cell co‐culture conditions. B, Schematic of the transwell system. Isolated B cells were seeded into a 24‐well plate and cultured with medium containing C‐176 (0.5 µM), while isolated T cells were inoculated into transwell chambers with 0.4 µm polycarbonate membranes. Cells were co‐cultured for 7 d and observed by fluorescence microscopy. Scale bard, 200 μm