FIGURE 6.

SNAI2 knockdown shows that IGFBP2 of the IGFBP family is downregulated in KLM1, KMP5, and Sphs. A, qRT‐PCR (IGFBP1 and IGFBP2) of control vector versus shSNAI2 from KLM1, KMP5, and Sphs. n = 3, each. ND, Not Detected. Mean + SE. **P < .01. B, Immunohistochemical IGFBP2 staining of human pancreatic cancer (2 samples). Scale bars: 100 µm. C, Western blot (IGFBP2 and ACTB) of control vector versus shSNAI2 from KLM1, KMP5, and Sphs. shRNA was used shSNAI2_1. The relative intensity (ratio) is shown below each band. D, E, Sphere formation assay in KLM1 after adding anti‐IGFBP2 neutralizing antibody (AF674) at day 7 compared with Vehicle. D, Microscope images. Scale bars: 100 µm. E, Quantification of number of spheres. n = 3, each. Mean + SE. *P < .05