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Schematic of NMJ chamber with primary SKM innervated by WT iPSC-MNs and electrically stimulated by electrodes for either direct (SKM-side) or indirect (MN-side) stimulation. Phase images indicate the morphology of co-culture on SKM innervated by axons progressing through tunnels (right) and WT iPSC derived MNs (left). Scale bars = 100 microns.
