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. 2021 Nov 22;9:745897. doi: 10.3389/fcell.2021.745897

FIGURE 3.

FIGURE 3

Anti-nAChR IgG effects on the NMJ. (A) A dose response curve established from NMJ stability of SKM dosed with the anti-nAChR Ab (diamond) from 0 to 20 μg/mL resulting in an IC50 of 3.4 μg/mL (N = 2, 3–10 replicates). A non-specific IgG antibody (triangle) did not significantly alter NMJ stability at 2 and 10 μg/mL (N = 2, 7–8 replicates). (B) ICC images of SKM revealing the antibody-antigen complex with the binding of anti-nAChR Ab (red channel) to the cell surface near the endplate marked by BunTX (green channel), overlay of red and green channels (upper left) and phase image with DAPI (lower right). Scale bars = 50 microns. (C) The number of contracting SKM under direct stimulation at dosing concentrations from 0 to 20 μg/mL (N = 2, 3–10 replicates). Statistics: One-way ANOVA followed by Dunnett’s test (alpha = 0.05) ∗∗p < 0.020, ∗∗∗p < 0.002. (D) ICC images of SKM with anti-nAChR Ab staining (red channel), sodium channel (Pan, green channel), overlay of red and green channels (upper left) and phase image on lower right. Scale bars = 50 microns.