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. 2021 Nov 22;9:745897. doi: 10.3389/fcell.2021.745897

FIGURE 5.

FIGURE 5

Direct effects of hCS and anti-nAChR on SKM. (A) Demonstrates the number of contracting myotubes under direct stimulation after dosing with 0.05% hCS (N = 2, 8 replicates), non-specific IgG (2 μg/mL, N = 3, 8 replicates), anti-nAChR-Ab (2 μg/mL, N = 2, 10 replicates) and a combination of 0.05% hCS and 2 μg/mL anti-nAChR-Ab (N = 2, 9 replicates). (B) ICC images of SKM incubated with 2 μg/mL of anti-nAChR with 0.05% hCS for 3 h and stained with anti-C3 (red channel) and BunTX (green channel). Scale bars = 50 microns.