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. 2021 Nov 22;12:779709. doi: 10.3389/fimmu.2021.779709

Figure 1.

Figure 1

Macroscopic isolation of TALT from mice head. (A) A stereomicroscopic image of the face around the eye of the mouse is shown. The nasolacrimal duct contains Evans blue that is a blue staining solution given via eye drops. The right panel shows the highly magnified image of the area surrounded by the white line in the left panel. (B) A whole-mount immunohistochemical image of the same sample in panel (A) is shown. B lymphocytes were detected with HRP labeled anti-B220 antibody (brown). The right image is an enlarged view of the squared area in the left panel. Each arrowhead in panels (A, B) shows a lacrimal sac of the nasolacrimal duct. (C–E) Hematoxylin and eosin-stained sections of excised ocular mucosal tissue. Panel (D) shows a highly magnified image of the lacrimal sac area surrounded by a black line in panel (C) An arrowhead indicates a lymphoid follicle of TALT. TALT is covered with stratified squamous epithelium. n = at least 5 animals. Bars: 500 µm (C), 100 µm (D), 20 µm (E).