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. 2021 Nov 22;11:761979. doi: 10.3389/fonc.2021.761979

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Copyright © 2021 Jin, Zhang, Yu, Zeng, Song, Huang, Kong, Hu and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LncRNA-ZEB1-AS1 expression is elevated in PC tissues and associated with PC progression. (A) Hierarchical clustering assessment of lncRNAs differentially expressed between normal pancreatic (NP) and pancreatic cancer (PC) tissues, which include lncRNA-ZEB1-AS1 (ZEB1-AS1). (B) ZEB1-AS1 expression was further compared among HPDC cells and the BxPC-3, PANC-1, SW1990, MIAPaCa, and ASPC-3 cell lines. (C) Single-molecule RNA FISH detection of ZEB1-AS1 (red) in indicated PANC-1/BxPC-3 cells. DAPI-stained nuclei were blue. (D) The expression level of ZEB1-AS1 in the subcellular fractions of BxPC-3 and PANC-1 cells was detected by qRT-PCR. The products of qRT-PCR were then separated by 2% agarose gel electrophoresis; U6 and β-actin were used as markers of the nucleus and cytoplasm, respectively. (E) Genomic location of ZEB1-AS1 and ZEB1 with transcript orientation marked by arrows. (F) ZEB1-AS1 expression was assessed by qRT-PCR in 119 PC and paired NP tissues. (G) RNA synthesis in PANC-1 cells was blocked by α-amanitin (50 µM), and ZEB1-AS1 stability was assessed by qRT-PCR compared with 0 h RNA polymerases II and I were used to transcribe β-actin and 18S rRNA, respectively. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^* p < 0.05 and ^** p < 0.01 as indicated.