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. 2021 Nov 22;11:761979. doi: 10.3389/fonc.2021.761979

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Copyright © 2021 Jin, Zhang, Yu, Zeng, Song, Huang, Kong, Hu and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of ZB1-AS1 inhibited proliferation, migration, and invasion of PC cells. (A) ZEB1AS1 inhibiting efficiency in BxPC-3 and PANC-1 cells were analyzed by qRT-PCR. (B) Proliferation of BxPC-3/PANC-1 cells underexpressing ZEB1-AS1 or negative control was assessed via MTT assays for 5 days. (C) Migration ability was evaluated by wound-healing assay. Representative images (left) and relative wound size are shown (right). (D) Transwell assay (left) was designed to assess the invasion ability of those transfected BxPC-3/PANC-1 cells. Average counts of five random microscopic fields were analyzed. The histogram (right) shows the percentage of invaded PC cell number. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^* p < 0.05 and ^** p < 0.01 as indicated.