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. 2021 Nov 22;11:761979. doi: 10.3389/fonc.2021.761979

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Copyright © 2021 Jin, Zhang, Yu, Zeng, Song, Huang, Kong, Hu and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZEB1 is a critical target of ZEB1-AS1 exerting functions in PC cells during hypoxia condition. (A) Expression of ZEB1 in BxPC-3/PANC-1 cells cultured with hypoxia medium for 0, 24, and 48 h were analyzed at the mRNA (left) and protein (right) levels by qRT-PCR and Western blot analysis, respectively. (B) Expression of ZEB1 in BxPC-3/PANC-1 cells transfected with ZEB1-AS1 siRNAs (si-ZEB1-AS1#1, si-ZEB1-AS1#2) or the negative control siRNA (siNC) was analyzed at the mRNA (left) and protein (right) levels by qRT-PCR and Western blot analysis, respectively. (C) Expression of ZEB1 in BxPC-3/PANC-1 cells transfected with ZEB1-AS1 overexpression plasmid (ZEB1-AS1) or the empty vector plasmid (Vector) was analyzed at the mRNA (left) and protein (right) levels by qRT-PCR and Western blot analysis, respectively. (D) ZEB1 in ZEB1-AS1 overexpressing BxPC-3/PANC-1 cells cultured in normal or hypoxia condition was analyzed at the mRNA and protein levels by qRT-PCR and Western blot analysis, respectively. (E, F) Migration and invasion ability of four groups of BxPC-3/PANC-1 cells were recorded by the Wound healing assay and Transwell assay, representative images were shown (left). Average counts of five random microscopic fields were analyzed. The histogram (right) shows the percentage of migrated or invaded PC cells number. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^* p < 0.05 and ^** p < 0.01 as indicated.