Fig. 6.

KDO25 alters macrophage migration.

(A) C57BL/6 mice were treated with TAA for 6 weeks or treated with TAA for 6 weeks followed by 2 weeks of co-administration with vehicle or KD025. Livers lysates were analysed for the level of pCofilin and actin. A representative immunoblot and densitometric analysis of pCofilin normalized to actin is shown (n = 6–11 animals/group). Data are presented as mean ± SEM.  ∗∗∗p <0.001 Mann–Whitney U test. (B) BMDM were treated with 10 μM KD025 or with DMSO (veh) for 20 min. Levels of pCofilin and GAPDH were determined by immunoblot. A representative blot and densitometric analysis are shown (n = 2 animals/group). (C) Mice were treated as in (A), and liver sections were analysed by histochemical staining for total cofilin. (D) BMDM were treated with KD025 (2 μM) of DMSO (veh) for 6 h, and total cofilin and GAPDH levels were assessed by immunoblot. A representative blot and densitometric analysis of cofilin normalized to GAPDH is shown (n = 2 animals/group). (E) Migration of BMDM in the presence of DMSO (veh) or 2 μM KD025 was assessed over 12 h by scratch wound assay and Incucyte microscopic analysis (n = 3 animals/group). Representative images at 8 h are shown. BMDM, bone marrow-derived macrophages; pCofilin, phosphorylated cofilin; O.D., optical density; TAA, thioacetamide.