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. 2021 Nov 22;12:764501. doi: 10.3389/fimmu.2021.764501

Figure 4.

Figure 4

Heat map and bioinformatics analysis flowchart. (A) Heat map of the similarity percentage of 26 H. capsulatum antigenic proteins against P. brasiliensis, B. dermatitidis, C. immitis, S. schenckii, A. fumigatus, C. neoformans, C. albicans, C. auris and H. sapiens and bioinformatics analysis flowchart. The analysis was performed by BLAST-p algorithm and ClustalX 2.1 software and the map plotted using software R package ggplot. (B) (I) Prediction of ligands to MHC-I was performed using NetMHC 4.0 server from the sequences of the 26 antigenic proteins previously described. (II) The NetMHCII 2.2 Server was used to predict MHC-II ligands, including the peptide sequences obtained by immunoprecipitation and that had low or no identity to human protein. (III) The Class I Immunogenicity software was used to predict the immunogenicity of the class I peptide MHC complex. (IV) The prediction of immunogenicity for MHC-II was performed by CD4+ T cell immunogenicity prediction server. (V) The IFNepitope server was used to predict IFN-γ induction by MHC-II binding peptides. HCDG_06427: Uncharacterized protein, HCDG_00690: Mitochondrial thiamine pyrophosphate carrier 1, HCBG_00112: Sm domain-containing protein, HCEG_08043: Ribosomal protein S23, HCDG_01939: Ribosomal protein L14, HCEG_00238: 60S ribosomal protein L27, HCDG_08240: 60S ribosomal protein L2, HCEG_04281: 60S ribosomal protein, HCBG_01774: 40S ribosomal protein S15, HCDG_01107: Peroxidase, HCBG_08832: HSP-60 like protein, HCEG_07158: H15 domain-containing protein, HCEG_09258: Glyceraldehyde-3-phosphate dehydrogenase, HCBG_06745: Fructose-bisphosphate aldolase, HCEG_02034: Enolase, HCAG_04686: ATP-dependent molecular chaperone HSC82, HCBG_01891: ATP synthase subunit alpha, HCEG_09064: Citrate synthase, HCBG_08684: Elongation factor 1-gamma, HCEG_08718: 6-Phosphogluconate dehydrogenase, HCAG_08367: Aldehyde dehydrogenase, HCAG_05721: Homogentisate 1,2-dioxygenase, HCBG_07920: Hsp70-like protein, HCEG_02400: Aha1_N domain-containing protein, HCBG_03027: 4-Hydroxyphenylpyruvate dioxygenase, HCBG_08743: Heat shock protein SSC1.