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. 2021 Nov 22;12:767136. doi: 10.3389/fphys.2021.767136

FIGURE 4.

FIGURE 4

Inhibition of Piezo1 channel suppressed expression of osteogenesis-related factors during OTM on the tension side. Immunohistochemical staining of RUNX2 (A), OSX (B), ALP (C), and COL1 (D) of PDL on the tension side of the distobuccal roots and the means of the integrated optical density (IOD) of RUNX2 (E), OSX (F), ALP (G), and COL1 (H). The expression of RUNX2, OSX, ALP, and COL1 in the OTM group increased significantly on days 3, 7, and 14 compared with that of the CON group, while it was significantly reduced by GsMTx4 on day 3 and day 7 for OSX, day 7 for RUNX2 and ALP, and day 7 and day 14 for COL1. (I) Western blot analyses were carried out to detect the protein levels of RUNX2, OSX, ALP, and COL1 on day 7 of OTM. GsMTx4 significantly reduced the protein expression of RUNX2, OSX, ALP, and COL1 on day 7 in the OTM + INH group. Data are presented as the mean ± SD. *p < 0.05, ***p < 0.001. Scale bar = 50 μm. RUNX2, Runt-related transcription factor 2; OSX, Osterix; ALP, alkaline phosphatase; COL1, collagen type 1; AB, alveolar bone; R, root.